Immunohistochemical staining:Article Title: mRNA and miRNA expression profiles in an ectoderm-biased substate of human pluripotent stem cells
Article Snippet: The primary antibodies used were as follows: mouse anti-keratan sulfate (R-10G) antibodies (IgG; monoclonal; 1:150 dilution; cat. no. RIT-M001; Cosmo Bio, Tokyo Japan), rabbit anti-vimentin antibodies (IgG; monoclonal; 1:300 dilution; cat. no. ab92547; Abcam, Cambridge, UK), mouse anti-vimentin antibodies (IgG; monoclonal; 1:100 dilution; cat. no. SC-6260; Santa Cruz Biotechnology, Santa Cruz, CA, USA), mouse anti-SSEA-4 antibodies (IgG; monoclonal; 1:300 dilution; cat. no. MAB4304; Merck, Darmstadt, Germany), mouse anti-TRA-1-60 antibodies (IgM; monoclonal; 1:300 dilution; cat. no. MAB4360; Merck), mouse anti-E-cadherin antibodies (IgG; monoclonal; 1:100 dilution; cat. no. MA1-10192; Thermo Fisher Scientific, MA, USA), mouse anti-SSEA-1 antibodies (IgM; monoclonal; 1:250 dilution; cat. no. MAB4301; Merck), mouse anti-OCT3/4 antibodies (IgG; monoclonal; 1:300 dilution; cat. no. sc-5279; Santa Cruz Biotechnology), rabbit anti-NANOG antibodies (IgG; monoclonal; 1:800 dilution; cat. no. D73G4; Cell Signaling Technology, Danvers, MA, USA), rabbit anti-ZEB1 antibodies (IgG; monoclonal; 1:150 dilution; cat. no. ab203829; Abcam), rabbit anti-SNAIL1/2 antibodies (IgG; polyclonal; 1:200 diultion; cat. no. ab180714; Abcam), rabbit anti-TWIST antibodies (IgG; polyclonal; 1:300 dilution; cat. no. ab50581; Abcam), rabbit anti-SOX17 antibodies (IgG; polyclonal; 1:50 dilution; cat. no. PA5-72230; Thermo Fisher Scientific), mouse anti-AFP antibodies (IgG; monoclonal; 1:100 dilution; cat. no. MAB-1368; R&D Systems, Minneapolis, MN, USA), rabbit anti-brachyury antibodies (IgG; monoclonal; 1:500 dilution; cat. no. ab209665; Abcam), mouse anti-αSMA antibodies (IgG; monoclonal; 1:50 dilution; cat. no. M0851; DAKO, Glostrup, Denmark), rabbit anti-OTX2 antibodies (IgG; monoclonal; 1:200 dilution; cat. no. 701948; Thermo Fisher Scientific), and mouse anti-TUJ1 antibodies (IgG; monoclonal; 1:500; cat. no. MMS-435P; BioLegend, San Diego, CA, USA).
Immunohistochemistry:Article Title: mRNA and miRNA expression profiles in an ectoderm-biased substate of human pluripotent stem cells
Article Snippet: The primary antibodies used were as follows: mouse anti-keratan sulfate (R-10G) antibodies (IgG; monoclonal; 1:150 dilution; cat. no. RIT-M001; Cosmo Bio, Tokyo Japan), rabbit anti-vimentin antibodies (IgG; monoclonal; 1:300 dilution; cat. no. ab92547; Abcam, Cambridge, UK), mouse anti-vimentin antibodies (IgG; monoclonal; 1:100 dilution; cat. no. SC-6260; Santa Cruz Biotechnology, Santa Cruz, CA, USA), mouse anti-SSEA-4 antibodies (IgG; monoclonal; 1:300 dilution; cat. no. MAB4304; Merck, Darmstadt, Germany), mouse anti-TRA-1-60 antibodies (IgM; monoclonal; 1:300 dilution; cat. no. MAB4360; Merck), mouse anti-E-cadherin antibodies (IgG; monoclonal; 1:100 dilution; cat. no. MA1-10192; Thermo Fisher Scientific, MA, USA), mouse anti-SSEA-1 antibodies (IgM; monoclonal; 1:250 dilution; cat. no. MAB4301; Merck), mouse anti-OCT3/4 antibodies (IgG; monoclonal; 1:300 dilution; cat. no. sc-5279; Santa Cruz Biotechnology), rabbit anti-NANOG antibodies (IgG; monoclonal; 1:800 dilution; cat. no. D73G4; Cell Signaling Technology, Danvers, MA, USA), rabbit anti-ZEB1 antibodies (IgG; monoclonal; 1:150 dilution; cat. no. ab203829; Abcam), rabbit anti-SNAIL1/2 antibodies (IgG; polyclonal; 1:200 diultion; cat. no. ab180714; Abcam), rabbit anti-TWIST antibodies (IgG; polyclonal; 1:300 dilution; cat. no. ab50581; Abcam), rabbit anti-SOX17 antibodies (IgG; polyclonal; 1:50 dilution; cat. no. PA5-72230; Thermo Fisher Scientific), mouse anti-AFP antibodies (IgG; monoclonal; 1:100 dilution; cat. no. MAB-1368; R&D Systems, Minneapolis, MN, USA), rabbit anti-brachyury antibodies (IgG; monoclonal; 1:500 dilution; cat. no. ab209665; Abcam), mouse anti-αSMA antibodies (IgG; monoclonal; 1:50 dilution; cat. no. M0851; DAKO, Glostrup, Denmark), rabbit anti-OTX2 antibodies (IgG; monoclonal; 1:200 dilution; cat. no. 701948; Thermo Fisher Scientific), and mouse anti-TUJ1 antibodies (IgG; monoclonal; 1:500; cat. no. MMS-435P; BioLegend, San Diego, CA, USA).
Expressing:Article Title: mRNA and miRNA expression profiles in an ectoderm-biased substate of human pluripotent stem cells
Article Snippet: The primary antibodies used were as follows: mouse anti-keratan sulfate (R-10G) antibodies (IgG; monoclonal; 1:150 dilution; cat. no. RIT-M001; Cosmo Bio, Tokyo Japan), rabbit anti-vimentin antibodies (IgG; monoclonal; 1:300 dilution; cat. no. ab92547; Abcam, Cambridge, UK), mouse anti-vimentin antibodies (IgG; monoclonal; 1:100 dilution; cat. no. SC-6260; Santa Cruz Biotechnology, Santa Cruz, CA, USA), mouse anti-SSEA-4 antibodies (IgG; monoclonal; 1:300 dilution; cat. no. MAB4304; Merck, Darmstadt, Germany), mouse anti-TRA-1-60 antibodies (IgM; monoclonal; 1:300 dilution; cat. no. MAB4360; Merck), mouse anti-E-cadherin antibodies (IgG; monoclonal; 1:100 dilution; cat. no. MA1-10192; Thermo Fisher Scientific, MA, USA), mouse anti-SSEA-1 antibodies (IgM; monoclonal; 1:250 dilution; cat. no. MAB4301; Merck), mouse anti-OCT3/4 antibodies (IgG; monoclonal; 1:300 dilution; cat. no. sc-5279; Santa Cruz Biotechnology), rabbit anti-NANOG antibodies (IgG; monoclonal; 1:800 dilution; cat. no. D73G4; Cell Signaling Technology, Danvers, MA, USA), rabbit anti-ZEB1 antibodies (IgG; monoclonal; 1:150 dilution; cat. no. ab203829; Abcam), rabbit anti-SNAIL1/2 antibodies (IgG; polyclonal; 1:200 diultion; cat. no. ab180714; Abcam), rabbit anti-TWIST antibodies (IgG; polyclonal; 1:300 dilution; cat. no. ab50581; Abcam), rabbit anti-SOX17 antibodies (IgG; polyclonal; 1:50 dilution; cat. no. PA5-72230; Thermo Fisher Scientific), mouse anti-AFP antibodies (IgG; monoclonal; 1:100 dilution; cat. no. MAB-1368; R&D Systems, Minneapolis, MN, USA), rabbit anti-brachyury antibodies (IgG; monoclonal; 1:500 dilution; cat. no. ab209665; Abcam), mouse anti-αSMA antibodies (IgG; monoclonal; 1:50 dilution; cat. no. M0851; DAKO, Glostrup, Denmark), rabbit anti-OTX2 antibodies (IgG; monoclonal; 1:200 dilution; cat. no. 701948; Thermo Fisher Scientific), and mouse anti-TUJ1 antibodies (IgG; monoclonal; 1:500; cat. no. MMS-435P; BioLegend, San Diego, CA, USA).
Microarray:Article Title: mRNA and miRNA expression profiles in an ectoderm-biased substate of human pluripotent stem cells
Article Snippet: The primary antibodies used were as follows: mouse anti-keratan sulfate (R-10G) antibodies (IgG; monoclonal; 1:150 dilution; cat. no. RIT-M001; Cosmo Bio, Tokyo Japan), rabbit anti-vimentin antibodies (IgG; monoclonal; 1:300 dilution; cat. no. ab92547; Abcam, Cambridge, UK), mouse anti-vimentin antibodies (IgG; monoclonal; 1:100 dilution; cat. no. SC-6260; Santa Cruz Biotechnology, Santa Cruz, CA, USA), mouse anti-SSEA-4 antibodies (IgG; monoclonal; 1:300 dilution; cat. no. MAB4304; Merck, Darmstadt, Germany), mouse anti-TRA-1-60 antibodies (IgM; monoclonal; 1:300 dilution; cat. no. MAB4360; Merck), mouse anti-E-cadherin antibodies (IgG; monoclonal; 1:100 dilution; cat. no. MA1-10192; Thermo Fisher Scientific, MA, USA), mouse anti-SSEA-1 antibodies (IgM; monoclonal; 1:250 dilution; cat. no. MAB4301; Merck), mouse anti-OCT3/4 antibodies (IgG; monoclonal; 1:300 dilution; cat. no. sc-5279; Santa Cruz Biotechnology), rabbit anti-NANOG antibodies (IgG; monoclonal; 1:800 dilution; cat. no. D73G4; Cell Signaling Technology, Danvers, MA, USA), rabbit anti-ZEB1 antibodies (IgG; monoclonal; 1:150 dilution; cat. no. ab203829; Abcam), rabbit anti-SNAIL1/2 antibodies (IgG; polyclonal; 1:200 diultion; cat. no. ab180714; Abcam), rabbit anti-TWIST antibodies (IgG; polyclonal; 1:300 dilution; cat. no. ab50581; Abcam), rabbit anti-SOX17 antibodies (IgG; polyclonal; 1:50 dilution; cat. no. PA5-72230; Thermo Fisher Scientific), mouse anti-AFP antibodies (IgG; monoclonal; 1:100 dilution; cat. no. MAB-1368; R&D Systems, Minneapolis, MN, USA), rabbit anti-brachyury antibodies (IgG; monoclonal; 1:500 dilution; cat. no. ab209665; Abcam), mouse anti-αSMA antibodies (IgG; monoclonal; 1:50 dilution; cat. no. M0851; DAKO, Glostrup, Denmark), rabbit anti-OTX2 antibodies (IgG; monoclonal; 1:200 dilution; cat. no. 701948; Thermo Fisher Scientific), and mouse anti-TUJ1 antibodies (IgG; monoclonal; 1:500; cat. no. MMS-435P; BioLegend, San Diego, CA, USA).
Construct:Article Title: mRNA and miRNA expression profiles in an ectoderm-biased substate of human pluripotent stem cells
Article Snippet: The primary antibodies used were as follows: mouse anti-keratan sulfate (R-10G) antibodies (IgG; monoclonal; 1:150 dilution; cat. no. RIT-M001; Cosmo Bio, Tokyo Japan), rabbit anti-vimentin antibodies (IgG; monoclonal; 1:300 dilution; cat. no. ab92547; Abcam, Cambridge, UK), mouse anti-vimentin antibodies (IgG; monoclonal; 1:100 dilution; cat. no. SC-6260; Santa Cruz Biotechnology, Santa Cruz, CA, USA), mouse anti-SSEA-4 antibodies (IgG; monoclonal; 1:300 dilution; cat. no. MAB4304; Merck, Darmstadt, Germany), mouse anti-TRA-1-60 antibodies (IgM; monoclonal; 1:300 dilution; cat. no. MAB4360; Merck), mouse anti-E-cadherin antibodies (IgG; monoclonal; 1:100 dilution; cat. no. MA1-10192; Thermo Fisher Scientific, MA, USA), mouse anti-SSEA-1 antibodies (IgM; monoclonal; 1:250 dilution; cat. no. MAB4301; Merck), mouse anti-OCT3/4 antibodies (IgG; monoclonal; 1:300 dilution; cat. no. sc-5279; Santa Cruz Biotechnology), rabbit anti-NANOG antibodies (IgG; monoclonal; 1:800 dilution; cat. no. D73G4; Cell Signaling Technology, Danvers, MA, USA), rabbit anti-ZEB1 antibodies (IgG; monoclonal; 1:150 dilution; cat. no. ab203829; Abcam), rabbit anti-SNAIL1/2 antibodies (IgG; polyclonal; 1:200 diultion; cat. no. ab180714; Abcam), rabbit anti-TWIST antibodies (IgG; polyclonal; 1:300 dilution; cat. no. ab50581; Abcam), rabbit anti-SOX17 antibodies (IgG; polyclonal; 1:50 dilution; cat. no. PA5-72230; Thermo Fisher Scientific), mouse anti-AFP antibodies (IgG; monoclonal; 1:100 dilution; cat. no. MAB-1368; R&D Systems, Minneapolis, MN, USA), rabbit anti-brachyury antibodies (IgG; monoclonal; 1:500 dilution; cat. no. ab209665; Abcam), mouse anti-αSMA antibodies (IgG; monoclonal; 1:50 dilution; cat. no. M0851; DAKO, Glostrup, Denmark), rabbit anti-OTX2 antibodies (IgG; monoclonal; 1:200 dilution; cat. no. 701948; Thermo Fisher Scientific), and mouse anti-TUJ1 antibodies (IgG; monoclonal; 1:500; cat. no. MMS-435P; BioLegend, San Diego, CA, USA).
Derivative Assay:Article Title: mRNA and miRNA expression profiles in an ectoderm-biased substate of human pluripotent stem cells
Article Snippet: The primary antibodies used were as follows: mouse anti-keratan sulfate (R-10G) antibodies (IgG; monoclonal; 1:150 dilution; cat. no. RIT-M001; Cosmo Bio, Tokyo Japan), rabbit anti-vimentin antibodies (IgG; monoclonal; 1:300 dilution; cat. no. ab92547; Abcam, Cambridge, UK), mouse anti-vimentin antibodies (IgG; monoclonal; 1:100 dilution; cat. no. SC-6260; Santa Cruz Biotechnology, Santa Cruz, CA, USA), mouse anti-SSEA-4 antibodies (IgG; monoclonal; 1:300 dilution; cat. no. MAB4304; Merck, Darmstadt, Germany), mouse anti-TRA-1-60 antibodies (IgM; monoclonal; 1:300 dilution; cat. no. MAB4360; Merck), mouse anti-E-cadherin antibodies (IgG; monoclonal; 1:100 dilution; cat. no. MA1-10192; Thermo Fisher Scientific, MA, USA), mouse anti-SSEA-1 antibodies (IgM; monoclonal; 1:250 dilution; cat. no. MAB4301; Merck), mouse anti-OCT3/4 antibodies (IgG; monoclonal; 1:300 dilution; cat. no. sc-5279; Santa Cruz Biotechnology), rabbit anti-NANOG antibodies (IgG; monoclonal; 1:800 dilution; cat. no. D73G4; Cell Signaling Technology, Danvers, MA, USA), rabbit anti-ZEB1 antibodies (IgG; monoclonal; 1:150 dilution; cat. no. ab203829; Abcam), rabbit anti-SNAIL1/2 antibodies (IgG; polyclonal; 1:200 diultion; cat. no. ab180714; Abcam), rabbit anti-TWIST antibodies (IgG; polyclonal; 1:300 dilution; cat. no. ab50581; Abcam), rabbit anti-SOX17 antibodies (IgG; polyclonal; 1:50 dilution; cat. no. PA5-72230; Thermo Fisher Scientific), mouse anti-AFP antibodies (IgG; monoclonal; 1:100 dilution; cat. no. MAB-1368; R&D Systems, Minneapolis, MN, USA), rabbit anti-brachyury antibodies (IgG; monoclonal; 1:500 dilution; cat. no. ab209665; Abcam), mouse anti-αSMA antibodies (IgG; monoclonal; 1:50 dilution; cat. no. M0851; DAKO, Glostrup, Denmark), rabbit anti-OTX2 antibodies (IgG; monoclonal; 1:200 dilution; cat. no. 701948; Thermo Fisher Scientific), and mouse anti-TUJ1 antibodies (IgG; monoclonal; 1:500; cat. no. MMS-435P; BioLegend, San Diego, CA, USA).
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